Journal: Development (Cambridge, England)

Article Title: Evolutionarily conserved requirement of Cdx for post-occipital tissue emergence.

doi: 10.1242/dev.079848

Figure Lengend Snippet: Fig. 5. Addition of Fgf8 to the whole embryo culture medium of Cdx2 null embryos rescues their axial elongation. (A,B)E8.0 (early somite) embryos at the start of the culture (A, wild type; B, Cdx2 null). (C-F)Embryos after their culture for the same period without (C,D) or with (E,F) Fgf8 added to the culture medium. C and E are controls; D and F are Cdx2 null mutants. (G-J)Mox1 expression in another set of Cdx2 null mutants (H,J) and controls (G,I) that have been cultured for the same period with (I,J) and without (G,H) Fgf8. (K)Comparison of posterior elongation of Cdx2 null and control embryos cultured without and with Fgf8. y axis, total number of somites generated in culture; bars on the graph represent median values; n, number of embryos; several experimental data are superimposed as one symbol in the graph as they had the same value. (L)Statistical analysis of the axial growth rescue of Cdx mutants by Fgf8 using the Mann-Whitney U test. al, allantois; flb, forelimb bud. Anterior is to the right in A,B and up in C-J. Scale bars: 0.5 mm. See also supplementary material Fig. S3.

Article Snippet: Recombinant Fgf8 (isoform b) was purchased from R&D Systems (423- F8).

Techniques: Embryo Culture, Expressing, Cell Culture, Comparison, Control, Generated, MANN-WHITNEY

Journal: Cell Metabolism

Article Title: GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans

doi: 10.1016/j.cmet.2018.12.016

Figure Lengend Snippet: GDF15 Expression Is Regulated by the Cellular ISR Pathway (A and C) GDF15 mRNA expression (A) and immunoblot analysis (C) of ISR components in wild-type (WT) mouse embryonic fibroblasts (MEFs) treated with vehicle control (Con), cobalt chloride (CoCl2, 625 μM), thapsigargin (Tg, 1 μM), tunicamycin (Tn 5 μg/mL), or L-Histidinol (His, 1 mM) for 6 h. (B) GDF15 mRNA expression in human cell lines (HeLA, HuH7, and A549) treated with Tn (5 μg/mL) for 6 h. (D) GDF15 mRNA expression in WT MEFs pre-treated for 1 h either with the PERK inhibitor GSK2606414 (GSK, 200 nM) or eIF2α inhibitor ISRIB (ISR, 100 nM), then co-treated with Tn (5 μg/mL) for a further 6 h. (E–G) GDF15 mRNA expression (E) in EIF2α Ser51 (SS) or phospho mutant (AA) MEFs or (F) in ATF4 wild-type (WT) or ATF4 knockout (KO) MEFs and (G) in control siRNA and CHOP siRNA transfected WT MEFs treated with Tn (5 μg/mL) for 6 h. (H) Diagram outlining pathway by which GDF15 and FGF21 expression is regulated by TN. mRNA expression is presented as fold expression relative to its respective control treatment for each cell type (set at 1) or TN-treated samples (set as 100) with normalization to HPRT gene expression in MEFs and GAPDH in human cells. Data are expressed as mean ± SD from at least three independent experiments. ∗∗∗ p < 0.001 versus control (con) for (A) and (B), and versus TN stimulated for (D)–(G) by two-tailed Student’s t test. Blots shown are representative of three independent experiments with Calnexin used as a loading control. See also Figures S3 and .

Article Snippet: Mouse FGF21 was analyzed by FGF21 Quantakine ELISA (R&D Systems) following the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Control, Mutagenesis, Knock-Out, Transfection, Gene Expression, Two Tailed Test

Journal: Cell Metabolism

Article Title: GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans

doi: 10.1016/j.cmet.2018.12.016

Figure Lengend Snippet:

Article Snippet: Mouse FGF21 was analyzed by FGF21 Quantakine ELISA (R&D Systems) following the manufacturer’s instructions.

Techniques: Recombinant, SYBR Green Assay, Protease Inhibitor, Reverse Transcription, Western Blot, Injection, Enzyme-linked Immunosorbent Assay, Control, TaqMan Assay, Software, Sterility, Electrophoresis, Real-time Polymerase Chain Reaction

Journal: BMC Medicine

Article Title: The anti-aging factor Klotho protects against acquired long QT syndrome induced by uremia and promoted by fibroblast growth factor 23

doi: 10.1186/s12916-021-02209-9

Figure Lengend Snippet: Demographic data of patients with dialysis-dependent CKD by quartile of circulating FGF23 levels

Article Snippet: Adult 14-week-old male C57BL/6 J mice (Charles River Laboratories International Inc.) were used to study the effect of FGF23 on heart rhythm by a single-dose intraperitoneal injection of recombinant mouse FGF23 (40 μg/kg) (R&D Systems) during ECG recording.

Techniques:

Journal: BMC Medicine

Article Title: The anti-aging factor Klotho protects against acquired long QT syndrome induced by uremia and promoted by fibroblast growth factor 23

doi: 10.1186/s12916-021-02209-9

Figure Lengend Snippet: FGF23 induces prolonged ventricular repolarization in healthy mice. A ECG waveforms in wild-type healthy mice before (black line) and after (red line) FGF23 administration (40 μg/kg). B–F Time course of QRS interval ( B ), QT interval ( C ), QTc interval ( D ), JT interval ( E ), and T peak to T end (TpTe) interval ( F ) measures from ECG recordings after vehicle ( N = 5 mice) or FGF23 ( N = 9 mice) administration. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus baseline

Article Snippet: Adult 14-week-old male C57BL/6 J mice (Charles River Laboratories International Inc.) were used to study the effect of FGF23 on heart rhythm by a single-dose intraperitoneal injection of recombinant mouse FGF23 (40 μg/kg) (R&D Systems) during ECG recording.

Techniques:

Journal: BMC Medicine

Article Title: The anti-aging factor Klotho protects against acquired long QT syndrome induced by uremia and promoted by fibroblast growth factor 23

doi: 10.1186/s12916-021-02209-9

Figure Lengend Snippet: Macroscopic and biochemical parameters in the experimental CKD model and after treatment with recombinant Klotho

Article Snippet: Adult 14-week-old male C57BL/6 J mice (Charles River Laboratories International Inc.) were used to study the effect of FGF23 on heart rhythm by a single-dose intraperitoneal injection of recombinant mouse FGF23 (40 μg/kg) (R&D Systems) during ECG recording.

Techniques: Recombinant

Journal: BMC Medicine

Article Title: The anti-aging factor Klotho protects against acquired long QT syndrome induced by uremia and promoted by fibroblast growth factor 23

doi: 10.1186/s12916-021-02209-9

Figure Lengend Snippet: Klotho prevents Kv4.2 α-subunit downregulation in ventricular myocytes from Nfx and after FGF23 incubation. A mRNA expression of Kv4.2 channel subunit in Sham ( N = 9 mice), Nfx ( N = 8 mice), Sham+rKL ( N = 9 mice), and Nfx+rKL ( N = 7 mice) mice. B mRNA expression of Kv4.2 in neonatal ventricular cardiomyocytes incubated with vehicle ( N = 6 replicate wells), FGF23 ( N = 9 replicate wells), rKL ( N = 9 replicate wells), and FGF23 + rKL ( N = 11 replicate wells). Data are presented as mean ± SEM. * P < 0.05 vs. the corresponding control (Sham or vehicle-neonatal cardiomyocytes) and # P < 0.05 vs. FGF23-neonatal cardiomyocytes

Article Snippet: Adult 14-week-old male C57BL/6 J mice (Charles River Laboratories International Inc.) were used to study the effect of FGF23 on heart rhythm by a single-dose intraperitoneal injection of recombinant mouse FGF23 (40 μg/kg) (R&D Systems) during ECG recording.

Techniques: Incubation, Expressing, Control

Journal: BMC Medicine

Article Title: The anti-aging factor Klotho protects against acquired long QT syndrome induced by uremia and promoted by fibroblast growth factor 23

doi: 10.1186/s12916-021-02209-9

Figure Lengend Snippet: Diagram representing the involvement of FGF23-Klotho axis in acquired long QT syndrome. CKD, chronic kidney disease; FGF23, fibroblast growth factor 23; I tof , fast transient outward potassium current; kl/kl , Klotho hypomorphic mice; Kv4.2, K + channel 4.2 subunit; Nfx, nephrectomy; sKL, soluble Klotho; Tg-kl, Klotho overexpression mice

Article Snippet: Adult 14-week-old male C57BL/6 J mice (Charles River Laboratories International Inc.) were used to study the effect of FGF23 on heart rhythm by a single-dose intraperitoneal injection of recombinant mouse FGF23 (40 μg/kg) (R&D Systems) during ECG recording.

Techniques: Over Expression

Journal: bioRxiv

Article Title: Fgf9-Nolz-1-Wnt2 Signaling Axis Regulates Morphogenesis of the Lung

doi: 10.1101/2022.08.10.503529

Figure Lengend Snippet: (A) Treatment with rFgf10 (200 n /ml) of wild type explant lung culture for 48 hr. qRT-PCR shows that Nolz-1 and Wnt2 are not changed in the rFgf10 treated group compared to the vehicle control. Student’s t -test, P > 0.05, n = 4. Scale bar, 500 μm. (B) Treatment with rFgf9 (200 ng/ml) results in enlarged epithelia in wild-type explant lungs cultured for 48 hr. The qRT-PCR shows that Nolz-1 , Wnt2 and Lef1 mRNAs are increased in rFgf9 treated group in wild type lungs. Student’s t -test, * P < 0.05, ** P < 0.01, n = 4. Western blotting showed that rFgf9 treatment increases Nolz-1 protein by 99% in wild type lung culture compared to the vehicle-treated group. Student’s t -test, * P < 0.05, n = 3. (C) Working hypothesis. Nolz-1 controls the proliferation of mesenchymal cells and the growth of epithelial branches through the regulation of Wnt2 signaling in the early stages of the development of the lungs. In the late stages of development, Nolz-1 acts non-cell autonomously to regulate the development of epithelial cells through Wnt2 signaling. Fgf9 acts upstream to regulate Nolz-1 expression in developing lungs.

Article Snippet: The lung explants were cultivated with recombinant mouse Fgf9 protein (200 ng/ml, R & D Systems, Inc.) or Fgf10 protein (200 ng/ml, R & D Systems, Inc.).

Techniques: Quantitative RT-PCR, Control, Cell Culture, Western Blot, Expressing